农业机械学报2012，Vol.43Issue(9)：136-142,7.DOI:10.6041/j.issn.1000-1298.2012.09.026
高静压和热处理对蘑菇多酚氧化酶的钝化动力学分析
Effect of High Hydrostatic Pressure and Heat Treatment on PPO Inactivation and Kinetic Analysis
摘要
Abstract
The effect of high hydrostatic pressure ( HHP) treatment and heat treatment on the stability of Agaricus bisporus mushroom polyphenoloxidase (PPO) was studied- Residual activities of the PPO were investigated after HHP treatment at 100 - 1 600 MPa for 0. 2 -25 min and heat treatment at 55 ~ 80℃ for 0, 2 ~20 min. The HHP and heat inactivation kinetic of mushroom PPO were analyzed by first-order and biphasic model. The results showed that the effect of inactivation was improved with the pressure and temperature increasing, as well as the treatment time increasing. The residual activities were all higher than 88% when treated at pressure lower than 600 MPa, indicated that treatment at pressure lower than 600 MPa had limited influence on the activity of PPO. After HHP treatment at 800, 1 000, 1 200, 1 400 and 1 600 MPa for 25 min, the residual activity reduced to 66.42% , 52. 83, 27. 20% , 2. 20% and 0, 01% , respectively. The first-order model was used to analyze the survival curves of heat treatment. All of the coefficients of determination (R2 ) were higher than 0. 960, which proved that the first-order model fit the microbial inactivation kinetics curve well. However, as R2 were all higher than 0.982, the biphasic model was proved to be more suitable than the first-order model to describe the kinetics of PPO inactivation treated by HHP.关键词
蘑菇/多酚氧化酶/高静压/钝化/动力学Key words
Mushroom, Polyphenoloxidase/ High hydrostatic pressure, Inactivation/ Kinetics分类
轻工纺织引用本文复制引用
易建勇,姜斌,董鹏,王蓉蓉,胡小松,张燕..高静压和热处理对蘑菇多酚氧化酶的钝化动力学分析[J].农业机械学报,2012,43(9):136-142,7.基金项目
国家自然科学基金资助项目(30972067)和“十二五”国家科技支撑计划资助项目(2011AA100801) （30972067）
