农业生物技术学报2012，Vol.20Issue(8)：858-866,9.DOI:10.3969/j.issn.1674-7968.2012.08.002
利用大白猪×民猪F2资源群体开展瘦肉量全基因组关联研究
Genome Wide Association Study for Lean Meat Weight in A Large White ×Minzhu Intercross Population
摘要
Abstract
The high-density single nucleotide polymorphism (SNP) genotyping platform allows genome wide association studies (GWAS) in pigs. In this GWAS, 355 pigs from a Large White(Sus scrofa) xMinzhu(S. Scrofa) intercross population were genotyped using the Illumina Porcine SNP 60K BeadChip, and phenotyped for lean meat weight (LMW) after slaughtered at age of (240±7) d. Association tests between the trait and the SNPs were performed via GWAS using the mixed model and Regression-Genomic Control (GRAMMAR-GC) approach. This GWAS revealed 14 SNPs which showed chromosome-wide significant (P<9.63e-06, SSC1; P<2.37e-05, SSC2; P<1.56e-05, SSC14) association with LWM. On SSC1, two SNPs ALGA0010777 and ALGA0010788, which were located at 285 030 256 and 285 276 856 bp, showed significant association with LWM. Ten significant SNPs were mapped to distal end of SSC2, where the known major gene IGF2 for muscle mass QTL was located. The remainder two SNPs ASGA0065444 and ASGA0065455, which were located at 99 627 980 and 100 078 535 bp on SSC14, were associated with the LWM significantly. The present GWAS results revealed SNPs and candidate genes for LWM in pigs, and the molecular mechanism of LWM difference between Chinese indigenous pig breed and Western commercial pig breed is initially clarified.关键词
全基因组关联研究/瘦肉量/猪Key words
Genome wide association study(GWAS)/ Lean meat weight/ Pig引用本文复制引用
程笃学,刘欣,李稳,梁晶,赵克斌,王立贤,罗维真,张龙超,颜华,李勇,王立刚,宋欣,马小军,陈少康..利用大白猪×民猪F2资源群体开展瘦肉量全基因组关联研究[J].农业生物技术学报,2012,20(8):858-866,9.基金项目
国家转基因生物新品种培育重大专项(No.2011ZX08006-003) （No.2011ZX08006-003）
国家“十二五”科技支撑计划项目(No.2011BAD28B01) （No.2011BAD28B01）
现代农业产业技术体系和中国农业科学院基本科研业务专项(No.2011cj-5) （No.2011cj-5）
