农业生物技术学报2012，Vol.20Issue(8)：880-885,6.DOI:10.3969/j.issn.1674-7968.2012.08.005
猪锌转运蛋白基因的克隆及其在消化道组织中的分布
Molecular Cloning and Distribution of Pig Zinc Transporter Gene in Digestive Tract
摘要
Abstract
slc30A family (vertebrate cation diffusion facilitator family proteins) transport zinc from the cytoplasm to the lumen, thus decreasing the content of zinc in cytosol. Cloning primers were designed according to the cloning principle of homologous sequence in this paper, the slc30A l~slc30A 9 genes were cloned by RT-PCR, then compared cloning sequences of slc30A1~slc30A9 and protein amino acid coding by these gene sequences with other species for homology analysis, and we also studied the slc30Al~slc30A9 distribution in the pig tissue. The main results showed that: The sequence length of gene slc30Al~slc30A9 was about 1 044-2 301 bp all with a complete open reading frame (ORF), which medicated to encode 348~767 amino acid residues, respectively(GenBank accession No. FJ374262.1, EU825192.2, FJ358706.1, EU835903.1, FJ230771.1, FJ237622.1, FJ237623.1, FJ588029 and FJ164069.1, respectively), but slc30A8 was the only mutant. The homology analysis results of slc30A and ZnT (zinc transporter) protein amino acid nearly were the same, higher with human (Homo taurus) and cow (Bos taurus), but lower with Rat (Rattus nurvegicus) and Mouse(Mus musculus), and difference were present in the different isomers and its coding proteins. The results of tissue distribution suggested that slc30A8 was only found in the ileum, slc30A9 was found in the esophagus and all tract, while slc30A 1 and slc30A 6 were found in other tissue except in the ileum. The other isomers were not found in the ileum, but there were different degree distribution in other tissue. These results suggested that the difference of the slc30Al~ slc30A9 genes distribution in all tissues maybe related to its special function in pig tissue.关键词
猪/slc30A/克隆/序列分析/组织分布Key words
Pig/ slc30A/ Cloning/ Sequence analysis/ Distribution引用本文复制引用
王月影,臧猛,李宏基,郭豫杰,鲁维飞,查光明,李奎,杨国宇..猪锌转运蛋白基因的克隆及其在消化道组织中的分布[J].农业生物技术学报,2012,20(8):880-885,6.基金项目
国家转基因生物新品种培育重大专项(No.2011ZX08006-003)和农业部948重点项目(No.2011-G35) （No.2011ZX08006-003）
