农业生物技术学报2012，Vol.20Issue(8)：922-927,6.DOI:10.3969/j.issn.1674-7968.2012.08.011
猪miR-369靶基因肿瘤坏死抑制因子-α(TNF-α)的鉴定
Identification of Porcine Tumor Necrosis Factor-α (TNF-α) as the Target Gene of the miR-369
摘要
Abstract
microRNAs (miRNAs), a class of small ~22-nucleotide-long noncoding RNAs, emerge as key post-transcriptional regulators of gene expression in eukaryotic organisms and involve in a variety of cellular processes, such as cell proliferation, cell differentiation and apoptosis. In silico analysis of the tumor necrosis factor-a (TNF-α) and miR-369 showed that the 3'UTR region of porcine (Sus scrofa) TNF-α adenylate/uridylate-rich elements (AREs) contained two putative miR-369 target sites. The porcine TNF-α 3'UTR region was constructed into the dual-luciferase reporter vector, transfected in the porcine iliac artery endothelial cell (PIEC cell) line to measure the luciferase activity by dual-luciferase reporter assay. The results indicated that the luciferase activity of miR-369 mimics group was significantly lower than that of the control group (P<0.05). Nonetheless, there was not a significant differences between the inhibitors and the control groups (P=0.752). Our results demonstrated that the TNF-α should be the target gene of miR-369 and provides evidence for further research of post-transcriptional mechanisms of TNF-α which affect fat deposition.关键词
猪/miR-369/肿瘤坏死抑制因子-α(TNF-α)/双荧光素酶报告基因系统Key words
Pig/ miR-369/ Tumor necrosis factor-α (TNF-α)/ Dual-luciferase reporter assay system引用本文复制引用
母治平,邓娟,王滔,李明洲,李学伟..猪miR-369靶基因肿瘤坏死抑制因子-α(TNF-α)的鉴定[J].农业生物技术学报,2012,20(8):922-927,6.基金项目
国家转基因生物新品种培育重大专项(No.2009ZX08009155B和No.2008ZX08006003) （No.2009ZX08009155B和No.2008ZX08006003）
