农业生物技术学报2012，Vol.20Issue(9)：1035-1041,7.DOI:10.3969/j.issn.1674-7968.2012.09.007
我国部分地区奶牛乳房炎源大肠杆菌生物学特性及耐药性分析
Characterization and Antibiotic Resistance of Ninety-five Strains of Escherichia coli Isolated from Bovine with Mastitis in Some Regions of China
摘要
Abstract
The aim of the study was to investigate that characterization and antibiotic resistance of Escherichia coli isolated from bovine mastitis in some areas of China for raising therapeutic effect and decreasing drug residues. Milk samples of clinical and subclinical bovine mastitis were collected from seven provinces in China and a total of 95 E. Coli isolates were further identified by microbiological tests. Following, serotype, pathogenicity and antibiotic resistance of 95 strains were also studied. The results showed that for the 95 E. Coli strains, 54 isolates were identified as 37 serogroups, 2 isolates were self-clumpinged and 39 isolates could not be serotyped, 093 and 09 serotypes were the most common serogroups. Obviously pathological changes were observed in internal organs of dissected experiment mouse (Mus musculus). Among the 95 strains, the rate of drug fast to 8 out of 16 antibacterial drugs exceeded 50%, and a same strain could resisted 14 kinds of antibacterial drugs at most and 2 kinds of antibacterial drugs at least. E. Coli strains which were resistant above 6 antibacterial drugs occupied 51.58%. So we concluded that serotypes of E. Coli isolates from bovine mastitis were complicate in China, and E. Coli isolates had produced different level resistance to many kinds of antibacterial drugs and presented serious multiple antibiotic resistance. This research provides a theoretical basis to further prepare bovine mastitis vaccine and treat bovine mastitis clinically.关键词
奶牛乳房炎/大肠杆菌/血清型/致病性/耐药性Key words
Bovine mastitis/ Escherichia coli/ Serotype/ Pathogenicity/ Antibiotic resistance引用本文复制引用
徐继英,刘俊林,李先波,霍生东,杨志强..我国部分地区奶牛乳房炎源大肠杆菌生物学特性及耐药性分析[J].农业生物技术学报,2012,20(9):1035-1041,7.基金项目
中央级公益性科研院所基本科研业务费专项资金项目(No.BRF070304)、甘肃省自然科学基金项目(No.3ZS061-A25-072)和中央高校基本科研业务费专项资金项目(No.zyz2011066) （No.BRF070304）
