农药学学报2012，Vol.14Issue(5)：497-502,6.DOI:10.3969/j.issn.1008-7303.2012.05.05
番茄灰霉病菌对咯菌腈的抗性诱变及抗药突变体的生物学特性
Induced mutation of Botrytis cinerea resistant to fludioxonil and biological characteristics of resistant mutants
摘要
Abstract
To assess the resistance risk of Botrytis cinerea to fludioxonil, the method for fludioxonil resistance mutagenesis and the biological characteristics of resistant mutants were studied. The results showed that the sub-lethal UV irradiation time for the conidia of B. Cinerea was 90-120 seconds. Four wild sensitive parental isolates were irradiated for sub-lethal time by UV, and six resistant mutants were obtained from two parental isolates. The ratio of EC50 value of resistant mutants comparing with that of its parental strain was more than 310 times,the frequency of resistance mutation was 3. 13×10-7. The mycelial growth rate, sporulation, sclerotia production and pathogenicity of resistant mutants decreased significantly as compared with their parental isolates. The analysis showed that the mutants had no cross resistance to other fungicides that have different mechanisms. The above results indicated that B. Cinerea on tomato had low risk of resistance to fludioxonil.关键词
番茄灰霉病菌/咯菌腈/抗性诱变/抗性突变体/抗药性风险Key words
Botrytis cinerea/ fludioxonil/ induced resistance mutation/ resistant mutants/ risk of resistance分类
农业科技引用本文复制引用
纪军建,张小风,王文桥..番茄灰霉病菌对咯菌腈的抗性诱变及抗药突变体的生物学特性[J].农药学学报,2012,14(5):497-502,6.基金项目
农业公益性行业(农业)专项(200903033). （农业）
