农药学学报2012，Vol.14Issue(5)：539-544,6.DOI:10.3969/j.issn.1008-7303.2012.05.12
茚虫威对映体在番茄、葡萄及水中的液相色谱-串联质谱检测方法
Determination of indoxacarb enantiomers in tomato, grape and water by liquid chromatography-tandem mass spectrometry
摘要
Abstract
A reversed-phase chiral method for the determination of indoxacarb enantiomers was established using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The target analytes were quantified by external standard method, and the difference in matrix effect between matrix dispersed extraction and solid phase extraction was compared. The tomato and grape samples were extracted by acetonitrile and then purified by NH2/Carb solid phase extraction (SPE) , while water was pre-concentrated and cleaned up by C18-H SPE cartridges. The chromatographic conditions were as follows:Chiralpak AD-RH column, V( acetonitrile): V(water) = 65:35, column temperature was 25 ℃, and flow rate was 0.4 mL/min. The results showed satisfied recoveries of the enantiomers in the range of 65. 5% -117.0% at levels from 0.005 mg/kg (or mg/L) to 0. 5 mg/kg (or mg/L) with relative standard deviations of4.1% -18.1% (n =5). Limits of detection (LOD) of the enantiomers were in the range of 1.4 -4.3μg/kg (or μg/L) ,and limits of quantification (LOQ) were 4. 7 -14.2 μg/kg (or μg/L). The method is simple and suitable for the routine analysis of indoxacarb enantiomers in tomato,grape and water.关键词
茚虫威/对映体/液相色谱-串联质谱/基质效应Key words
indoxacarb /enantiomer/LC-MS/MS/ matrix effect分类
化学化工引用本文复制引用
王欢庆,董丰收,刘新刚,徐军,李远播,孔志强,向文胜,郑永权..茚虫威对映体在番茄、葡萄及水中的液相色谱-串联质谱检测方法[J].农药学学报,2012,14(5):539-544,6.基金项目
国家自然科学基金(NSFC,31071706)资助项目. （NSFC,31071706）
