皮革科学与工程2012，Vol.22Issue(5)：9-14,6.
胶原与明胶的结构研究:方法、结果与分析
Study on Structures of Collagen and Gelatin:Methods, Results and Analysis
摘要
Abstract
Collagen and its denatured product, gelatin, can have the same origin, however, their structures and properties are significantly different. In the present study, the methods of ultraviolet spectroscopy (UV) , Fourier transform infrared spectroscopy ( FT - IR) , circular dichroism ( CD) , ultra - sensitive differential scanning calorimetry (US - DSC), Zeta potential, and atomic force microscopy (AFM) have been used to study structures of the collagen extracted from bovine tendon and the gelatin derived from the collagen. The results indicate that collagen and gelatin can not be distinguished by UV measurements. However, they show different FT - IR characterizations. CD directly indicates the triple helix conformation of collagen, while gelatin no such structural spectrum. The transition from triple helix to random coil of collagen can be sensitively monitored by US - DSC, meanwhile, gelatin has no such transition. Zeta potential method need to be further studied. And AFM observation reveals the fibrillar structure of collagen on mica; nevertheless gelatin presents different morphology. The present work is significant for structure investigation of protein.关键词
胶原/明胶/傅立叶变换红外光谱法/圆二色谱法/超灵敏差示扫描量热法/原子力显微镜Key words
collagen/ gelatin/ structure/ FT - IR/ CD/ US - DSC/ AFM分类
轻工纺织引用本文复制引用
朱欣星,安然,李昌朋,林炜..胶原与明胶的结构研究:方法、结果与分析[J].皮革科学与工程,2012,22(5):9-14,6.基金项目
国家自然科学基金(21074074)和四川省项目(2010HH0004) （21074074）
