青岛大学医学院学报2012，Vol.48Issue(5)：381-383,387,4.
ghrelin对糖尿病大鼠下丘脑弓状核胃牵张敏感神经元放电活动的影响
EFFECTS OF ghrelin ON DISCHARGE ACTIVITY OF GASTRIC DISTENTION NEURONS IN HYPOTHALAMIC ARCUATUS NUCLEUS OF DIABETIC RATS
摘要
Abstract
Objective To observe the changes of activity of gastric distention (GD) sensitive neurons of hypothalamic arcuate nucleus (Arc) in rat models with diabetes mellitus (DM) , and to study the effects of ghrelin on GD neurons in hypothalamic arcuatus nucleus in the rats. Methods A rat model of DM was created by intraperitoneal injection of streptozotocin (STZ). The effects of ghrelin and [D-Lys-3]-GHRP-6 on GD sensitive neurons of Arc in DM rats were observed by recording extracellular potentials of single neurons. Results In normal rats, 98 GD sensitivity neurons were recorded in Arc of normal rats, in which, 64. 3% were classified as GD-excitatory (GD-E) neurons, and 35. 7% were GD-inhibitory (GD-I) neurons. Microinjection of ghrelin could excite 73. 0% of GD-E neurons, and discharge frequency significantly increased (t = 2. 01 ,P<0. 05) , and inhibite 60. 0% of GD-I neurons, and lower their discharge frequency (i = 4. 49,P<0. 01). The effect of ghrelin could be blocked by antagonist of ghrelin-[D-Lys-3]-GHRP-6. In DM rats, 66 GD sensitivity neurons recorded in Arc, in which, 56. 1% were GD-E, and 43. 9% were GD-I. Microinjection of ghrelin could excite 35. 1% GD-E neurons, and discharge frequency significantly increased (t = 2. 67, P<0.05), and inhibit 72. 4% of GD-I neurons, and discharge frequency reduced (t = 3. 95,P<0. 01). Compared with normal rats, the ratio between GD-E and GD-I neurons in Arc of diabetic rats was not significantly changed (P>0. 05) , but ghrelin made neuronal excitation ratio of GD-E obviously reduce (x2 = 3. 86,P<0. 05) , and the average increase rate of discharge frequency was also significantly decreased (t = 2. 02,P<0. 05), the inhibition ratio and discharge average reduction rate reduced by ghrelin were not significantly changed in GD-I neurons (P>0. 05). Conclusion The ghrelin of hippocampus Arc involves in regulation of spontaneous discharge activity of GD sensitive neurons in diabetic rats, which is likely to be realized through ghrelin receptor.关键词
弓状核/ghrelin/糖尿病/胃牵张敏感神经元/大鼠Key words
arcuate nucleus/ ghrelin/ diabetes mellitus/ gastric distention sensitive neurons/ rats分类
医药卫生引用本文复制引用
侯滕菲,徐珞..ghrelin对糖尿病大鼠下丘脑弓状核胃牵张敏感神经元放电活动的影响[J].青岛大学医学院学报,2012,48(5):381-383,387,4.基金项目
国家自然科学基金项目(No.30470642,No.30670780,No.31071014,No.81100260,No.81070305) （No.30470642,No.30670780,No.31071014,No.81100260,No.81070305）
山东省科技攻关项目(No.2008GG10002006) （No.2008GG10002006）
山东省卫生厅项目(No.2007HZ026) （No.2007HZ026）
青岛市科技局项目(11-2-3-3-(2)-nsh) （11-2-3-3-(2）
