热带作物学报2012，Vol.33Issue(6)：981-987,7.DOI:10.3969/j.issn.1000-2561.2012.06.003
人工老化对红麻种子活力及基因组DNA的影响
The Effects of Artificial Aging on Kenaf Seed Vigor and the Genomic DNA
摘要
Abstract
The vigor changes and the genomic DNA integrity were studied among 3 kenaf(Hibiscus canrwbinus L.) seeds by artificial accelerated aging. Results showed that germination potential, germination rate, germination index and vigor index of these seeds declining along with seeds aging time on the condition of high temperature(44眑)t and high humidity (95% RH) , while the decreasing rate of vigor was dynamic and there were differences in 3 varieties. Through checking the genetic DNA integrity of the control and the different aging treatments of 3 materials by using selected 23 RAPD primers, the result showed that the allele frequencies of 83-20 and Tainong No.l were 100% without difference and Liao55 was 100.85% with difference, but the result of t-test were no significant difference, which indicated that artificial aging did not significantly affect genomic DNA of kenaf seeds, namely, aging had no significant effect on genetic integrity of kenaf germplasm.关键词
红麻/人工老化/种子活力/基因组DNAKey words
Kenaf/ Artificial aging/ Seed vigor/ Genomic DNA分类
农业科技引用本文复制引用
戴志刚,王凤敏,陈基权,粟建光,龚友才,温岚..人工老化对红麻种子活力及基因组DNA的影响[J].热带作物学报,2012,33(6):981-987,7.基金项目
农业部种质资源保护项目(NB07-2130135-39) （NB07-2130135-39）
中央级院所基本科研业务费专项(No.0032010039). （No.0032010039）
