热带作物学报2012，Vol.33Issue(6)：1057-1062,6.DOI:10.3969/j.issn.1000-2561.2012.06.017
香蕉后熟果皮差异蛋白分析及其基因克隆的研究
Analysis of Differentially Expressed Proteins and the Gene Cloning in Ripening Banana Peel
摘要
Abstract
Differentially expressed proteins were isolated by the two-dimensional (2D) gel electrophoresis, and analysed by the Image Master software, and identified by the mass spectrometry, after the extraction of the total protein of the control and the ethylene-induced early-ripening banana peel. A putative chitinase M (MaCH HI) was picked out for subsequent research from the differentially expressed proteins. The nucleic acid sequence to corresponding peptide sequence of MaCH HI was found by the NCBI BLAST. The full-length DNA sequence of MaCH IH gene was obtained by polymerase chain reaction (PCR) using PCR primers, which were designed according to the nucleic acid sequence. The results showed that this sequence share about 99% homology to a II class acidic chitinase in other species. And then, it was preliminarily confirmed as a gene of HI kind of acidic chitinase in banana.关键词
香蕉/后熟/乙烯诱导/差异蛋白/几丁质酶基因Key words
Banana/ Ripening/ Ethylene-induced/ Differentially expressed proteins/ Chitinase gene分类
农业科技引用本文复制引用
李艳霞,冯仁军,张丽丽,张银东..香蕉后熟果皮差异蛋白分析及其基因克隆的研究[J].热带作物学报,2012,33(6):1057-1062,6.基金项目
中央级公益性科研院所基本科研业务费项目"乙烯诱导的香蕉果皮差异表达蛋白的研究"(No.ITBB110204). （No.ITBB110204）
