热带作物学报2012，Vol.33Issue(6)：1063-1067,5.DOI:10.3969/j.issn.1000-2561.2012.06.018
转无机焦磷酸化酶基因甘蔗对根际土壤微生物群落多样性的影响
Effect of Inorganic Pyrophosphorylase Transgenic Sugarcane on Microbial Community Diversity in Rhizosphere Soil
摘要
Abstract
The effect of inorganic pyrophosphorylase (PPase) transgenic sugarcane on the microbial diversity in rhizosphere soil was studied by Biolog Eco plate method. The results showed that the Average Well Color Development (A WCD) of microbial community in the rhizosphere soil of transgenic sugarcane was higher than that of non-transgenic sugarcane, indicating the carbon utilization capability of the former was higher than that of the latter. The indices of Shannon (//), Smipson (1/D) and' Mclntosh (U) were calculated respectively to show the richness, dominance and evenness of the functional diversity accordingly. The results showed that the microbial diversity indices in the rhizosphere soil of the transgenic sugarcane were also higher, and the dominance and the evenness of the functional diversity were remarkably improved. Principal components analysis (PCA) revealed that there were significant differences of carbon substrate utilization patterns between transgenic sugarcane and non-transgenic sugarcane, as the main carbon sources of differentiation were carbohydrates, followed by fatty acids and lipids. It indicated that the transgenic sugarcane roots in the growing season might induce the development of certain physiological characteristics of the microbial community, and hence a change in the functional diversity of the soil microbial communities, and had relatively higher activity of metabolism.关键词
转基因甘蔗/土壤微生物/功能多样性Key words
Transgenic sugarcane/ Soil microbial community/ Functional diversity分类
农业科技引用本文复制引用
谭燕华,王俊刚,张树珍,王军,彭存智,易小平..转无机焦磷酸化酶基因甘蔗对根际土壤微生物群落多样性的影响[J].热带作物学报,2012,33(6):1063-1067,5.基金项目
中央级院所基本业务科研费项目(No.ITBB110604). （No.ITBB110604）
