热带作物学报2012，Vol.33Issue(6)：1084-1088,5.DOI:10.3969/j.issn.1000-2561.2012.06.022
中国水仙NtPI基因的克隆与表达分析
Cloning and Expression Analysis of NtPI Homologous Gene from Narcissus tazetta var. chinensis
摘要
Abstract
A flowering-related MADS-box gene named NtPI (GenBank accession JQ326272) was cloned from the flower bud of Narcissus tazetta var. chinensis using rapid amplification of cDNA ends. The cDNA is 804 bp in length with an open reading frame 618 bp which is capable of encoding 205 amino acids. The deduced amino acids sequence contained a typical MADS domain and a K domain. Homology analysis showed that NtPI shared 98% homology with Narcissus cyclamineus. Phylogenetic analysis indicated that NtPI belonged to the AP3/PI family PI lineage. RT-PCR revealed that the existence of NtPI was detectable in flowers, while the levels were much higher in stamen and pistil than in petal and corona from 'Jinzhanyintai', but the levels were much higher in petal and corona than in stamen and pistil from ' Yulinglong'.关键词
中国水仙/花器官发育/MADS-box基因/NtPI基因Key words
Narcissus tazetta var. chinensis/ Floral organ development/ MADS-box gene/ NtPI gene分类
农业科技引用本文复制引用
吴菁华,杨超,吴少华..中国水仙NtPI基因的克隆与表达分析[J].热带作物学报,2012,33(6):1084-1088,5.基金项目
国家科技支撑计划项目(No.2007BAD07B04) （No.2007BAD07B04）
福建省自然科学基金项目(No.2009J01066). （No.2009J01066）
