热带作物学报2012，Vol.33Issue(7)：1219-1224,6.DOI:10.3969/j.issn.1000-2561.2012.07.014
响应面法优化枇杷花总黄酮超声波辅助提取工艺的研究
Optimization of Ultrasonic Extraction for Flavonoids from Loquat Flowers with Response Surface Methodology
摘要
Abstract
This study aimed to optimize the ultrasonic extraction method for flavonoids from Loquat flowers with response surface methodology. Ultrasonic power, ethanol concentration, temperature, treatment time, ratio of solvent to material and treatment times to the extracting rate of flavonoids were studied. Based on the single factor experiments, 80 w of ultrasonic power and single extraction were chosen to further study. The interactive effects of four crucial parameters, including ethanol concentration, temperature, treatment time and ratio of solvent to material, were studied by central composite (Box 桞ehnken) and were assessed by response surface methodology (RSM). The results showed that the optimal preparation process of flavonoids extraction was 47.33% of ethanol concentration (V/V) and 45.56:1 of solvent to material rate (mL:g) at 58.42X1 for 12.01 min under the ultrasonic power of 80 w. At this condition, the extracting rate of flavonoids from Loquat flowers could be up to 10.48% for a single extraction.关键词
枇杷花/总黄酮/超声波提取/响应面分析Key words
Loquat flowers/ Flavonoids/ Ultrasonic extraction/ Response surface methodology分类
农业科技引用本文复制引用
周丹蓉,郑少泉,蒋际谋..响应面法优化枇杷花总黄酮超声波辅助提取工艺的研究[J].热带作物学报,2012,33(7):1219-1224,6.基金项目
公益性行业(农业)科研专项经费项目(No.201003073) （农业）
农业科技成果转化资金项目(No.2010GB2C400213) （No.2010GB2C400213）
农业部作物种质资源保护项目(NB2011-2130135-08) （NB2011-2130135-08）
农业部热带作物种质资源保护项目(12RZZY). （12RZZY）
