热带作物学报2012，Vol.33Issue(7)：1239-1244,6.DOI:10.3969/j.issn.1000-2561.2012.07.018
一株木薯生淀粉糖化酶菌株的分离及酶学性质研究
Isolation of a RSDG-producing Strain Aspergillus niger ITBB FC2 and Its Enzymatic Characterization
摘要
Abstract
Aspergillus niger strain ITBB FC2, isolated from decayed raw cassava root showed strong raw-starch-digrading-glucoamylase (RSDG) activity in liquid fermentation medium with 2% cassava flour. The crude enzyme had an activity of 495.04 U/mL and produced 970 mg/L of glucose in the 2% cassava flour fermentation medium when inculated at 37 °C, 200 r/min for 3 days, while it had an activity of 6 330 U/g in solid fermentation medium (bran:water=l: 1). The optimal pH was 4-5. 2 mmol/L. Ca2+ promoted the enzyme activity by 133.74%.关键词
黑曲霉/生淀粉糖化酶/酶学性质Key words
Aspergillus niger, Raw—starch—digrading—glucoamylase/ Enzymatic characterization分类
农业科技引用本文复制引用
钟坤,谭德冠,孙雪飘,彭明,张家明..一株木薯生淀粉糖化酶菌株的分离及酶学性质研究[J].热带作物学报,2012,33(7):1239-1244,6.基金项目
科技部国际科技合作计划项目“热带生物质能源高效转化及综合利用”(No.2010DFA62040). （No.2010DFA62040）
