四川大学学报（自然科学版）2012，Vol.49Issue(4)：895-900,6.DOI:10.3969/j.issn.0490-6756.2012.04.032
甘蓝型油菜Tic21基因克隆、表达及拷贝数鉴定
Isolation andexpression analysis of Brassica napus L.Tic21 gene and its copy number determination
摘要
Abstract
A cDNA sequence with primers based on Arabidopsis (Arabidopsis thaliana L. ) was obtained from Brassica napus L. , which was named as BnTic21 (GenBank accession number HQ613273) and could be delineated into 5 exons and 4 introns. An ORF (Open Reading Frame) of 885 bp encoded a 294 predicted amino acid residues with a molecular weight of 31. 24 kD, and an isoelectric point of 11. 10. The amino acid sequence similarity between the BnTic21 gene and some genes from other plants in Gen-Bank were 60. 40%~87. 75%. Real-time PCR was analyzed in different tissues. The results showed that the BnTic21 gene's expression level in various organs were found as follows; shoot apex> cotyledon> leaf> hypocotyl > root. Real-time PCR analysis showed that there were about two copies of Bn-Ttc21gene in the genome of Brassica napus L.关键词
甘蓝型油菜/Tic21/Real-time PCR/拷贝数Key words
Brassica napus L. / Tic21/ Real-time PCR/ copy number分类
农业科技引用本文复制引用
刘冠财,刘洋,任彩霞,张浩,王茂林..甘蓝型油菜Tic21基因克隆、表达及拷贝数鉴定[J].四川大学学报（自然科学版）,2012,49(4):895-900,6.基金项目
国家"863"(2009AA101105) （2009AA101105）
四川省十一五油菜育种攻关项目(2006yzgg) （2006yzgg）
