四川大学学报（自然科学版）2012，Vol.49Issue(4)：901-908,8.DOI:10.3969/j.issn.0490-6756.2012.04.033
二酰基甘油转移酶对三角褐指藻油脂积累及相关环境因子响应的研究
The response of diacylglycerol acyltransferase to lipid accumulation and related environmental factors in Phaeodacylum tricornutum
摘要
Abstract
A full-length diacylglycerol acyltransferase cDNA was isolated from Phaeodactylum tricornutum (Ptdgat) using RT-PCR and RACE methods. Sequence analysis indicated that this cDNA has an open reading frame of 1587bp, encoding 528 amino acids. A shuttle plasmid, named as pPha-Ptdgat RNAi, which contained a 460bp invert-repeat RNA construction based on the ORF of Ptdgat, was constructed according to the need for RNA interference. The transformants were obtained by gene gun, bi-olistic PDS-1000/He particle delivery system. Real-time PCR analysis revealed that there was a significantly lower expression level of Ptdgat in the transformants than in the wild diatom. And the analysis of lipid content showed there was a lower lipid content in the transformed algae than in the wild diatom. Furthermore, the Ptdgat expression level and the lipid content were increased when appropriate concentrations of iron, silicon or ABA was in media for P. tricornutum, respectively.关键词
三角褐指藻/二酰基甘油转移酶/RNAi/油脂积累/生物柴油Key words
Phaeodactylum tricornutum/ Diacylglycerol acyltransferase/ RNAi/ Hpid accumulation/ biodiesel分类
生物科学引用本文复制引用
凃丽,于广欣,陈波,曲芳兵,冷鹂,兰利琼,卿人韦..二酰基甘油转移酶对三角褐指藻油脂积累及相关环境因子响应的研究[J].四川大学学报（自然科学版）,2012,49(4):901-908,8.基金项目
国家高技术发展计划(863计划)(2007AA09Z427,2008AA10Z409) （863计划）
国家自然科学基金面上项目(40976092) （40976092）
横向项目(2010H0311) （2010H0311）
中央高校项目(2010SCU22006) （2010SCU22006）
