四川大学学报（自然科学版）2012，Vol.49Issue(4)：924-930,7.DOI:10.3969/j.issn.0490-6756.2012.04.037
ChiVMV四川分离物的分子鉴定及致病性初步研究
Identification and preliminary study on pathogenicity of Chilli veinal mottle virus obtained from Sichuan
摘要
Abstract
One isolate (Pp3) was isolated from infected pepper in Sichuan province. The isolate was identified as Chilli veinal mottle virus (ChiVMV). The CP (coat protein) gene of the isolate was cloned by RT-PCR (reverse transcription polymerase chain reaction) and was sequenced and compared with the CP gene sequences of other ChiVMVs reported in GenBank. The results showed that the sequence of the CP gene was 861 nucleotides in length. Sequence alignment showed that this isolate shared 80. 0%~88. 0% identity in the CP nucleotide sequence level with those ChiVMV isolates obtained from other parts of the world. Based on the phylogenetic analysis of the CP gene of ChiVMVs, it showed that the variation of i-solate Pp3 was evident. Using model plant Nicotiana tabacum the pathogenicity of this virus was studied preliminarily. The results revealed that Pp3 had higher pathagenicity and could induce systemic necrosis in N. tabacum.关键词
辣椒脉斑驳病毒/外壳蛋白基因/序列分析/致病性Key words
Chilli veinal mottle virus/coat protein gene/ sequence analysis/ pathogenicity分类
农业科技引用本文复制引用
贾树丹,王绍东,王建辉,林宏辉,席德慧..ChiVMV四川分离物的分子鉴定及致病性初步研究[J].四川大学学报（自然科学版）,2012,49(4):924-930,7.基金项目
国家自然科学基金(31071669,31171835) （31071669,31171835）
高校基本科研业务费(2011SCU04B34) （2011SCU04B34）
