首都师范大学学报（自然科学版）2012，Vol.33Issue(4)：27-31,5.
睡菜外植体愈伤组织诱导与培养初步研究
A Preliminary Study of Tissue Cultrue and Rapid Propagation on Menyanthes trifoliata L.
摘要
Abstract
The study takes fresh stem with bud from Menyanthes trifoliata as explants, then cut it into fragments about 0. 5cm long. Through a series of optimization expriment for cultural medium composition of phytohormones, the best culture medium formula for callus induction of this plant' s stem fragments was selected. The results indicated that the young stem segment with buds was the best explant; the suitable sterilization time with 75% alcohol was 30s and with 1 g·L-1 HgCl2 9 min. MS + BA 1.0 mg·L-1 + NAA 0. 10 mg·L-1 was the proper medium for primary culture, and MS + BA 0. 5 mg·L-1 + NAA 0. 05mg·L-1 was the proper medium for subculture. Adding 1. 5 g/L of carbendazim powder to the medium containing could inhibit the growth of fungi effectively. The callus should be timely subcultured in 30 days after callus has been inducted.关键词
睡菜/组织培养/愈伤组织Key words
Menyanthes trifoliata/tissue culture/callus分类
农业科技引用本文复制引用
吴昌宇,华振玲,李学东..睡菜外植体愈伤组织诱导与培养初步研究[J].首都师范大学学报（自然科学版）,2012,33(4):27-31,5.基金项目
北京市科委重大科技计划项目(D08040600580803)资助 （D08040600580803）
