山东中医药大学学报2012，Vol.36Issue(5)：439-442,4.
20种中药多糖水解成分HPLC指纹图谱与寒热药性关系的SVM分析研究
Study on relationship between cold-hot natures and HPLC fingerprints of polysaccharides of 20 traditional Chinese drugs based on SVM
摘要
Abstract
Objective:To discuss the correlation between cold-hot natures and polysaccharides,to reveal the relation of cold-hot natures and material basis. Methods: Extracting polysaccharides from 10 kinds of cold-property traditional Chinese drugs (TCDs) and 10 kinds of hot-property TCDs. The polysaccharides were completely hydrolyzed into monosaccharides with trifluoroacetic acid. The monosaccharide derivatives obtained with l-phenyl-3-methyl-5-pyra-zolone were separated by reverse-phase high performance liquid chromatography(RP-HPLC). The results of the HPLC fingerprints of monosaccharides composition of polysaccharides were analyzed by support vector machine(SVM) method. Results:SVM analysis could accurately distinguish cold-hot natures of the 20 kinds of TCDs,the correct rate was 100%. Conclusion:There was a significant correlation between cold-hot natures and polysaccharides. The SVM analysis could be used as statistical tools to distinguish the cold and hot natures. The polysaccharide was one of the material basis of cold-hot natures of TCDs.关键词
多糖/HPLC/指纹图谱/寒热药性/支持向量机Key words
polysaccharides/HPLC/fingerprint/cold-hot natures/support vector machine分类
医药卫生引用本文复制引用
王晓燕,李峰..20种中药多糖水解成分HPLC指纹图谱与寒热药性关系的SVM分析研究[J].山东中医药大学学报,2012,36(5):439-442,4.基金项目
国家重点基础研究发展计划(973计划)项目(编号:2007CB512601) （973计划）
