山东农业科学2012，Vol.44Issue(9)：85-89,5.
泡菜中两株乳酸菌的分离鉴定及其混合培养初探
Isolation and Identification of Two Strains of Lactic Acid Bacteria from Kraut and Preliminary Study on Their Co-culture
摘要
Abstract
Two strains of acidophilic Lactobacillus bacteria BL1 and BL2 were isolated from Kraut. After fermented for 24 hours in MRS medium, the two strains could make the pH value of the fermenting liquid below 3.5. Both bacteria were identified to be Lactobacillus sp. based on the morphological and physical characters. The 16S rDNA genes of both bacteria were cloned by PCR, and their accession number in NCBI were HM800503 and HM800504 respectively. The nucleotide sequences of 16S rDNA of BL1 and BL2 had the highest identity of 99% with L. pewlens and L. rhamnosus, respectively. The co - culture results showed that they could activate the growth of each other.关键词
泡菜/乳杆菌/菌种鉴定/混合培养Key words
Kraut/ Lactobacillus/ Strain identification/ Co-culture分类
生物科学引用本文复制引用
刘孝永,裘纪莹,孙欣,周庆新,祝清俊,王未名,陈蕾蕾..泡菜中两株乳酸菌的分离鉴定及其混合培养初探[J].山东农业科学,2012,44(9):85-89,5.基金项目
国家自然科学基金项目(31100039) （31100039）
中俄政府间合作项目“益生菌在果蔬防腐保鲜中的应用技术研究”. （）
