实验动物与比较医学2012，Vol.32Issue(4)：295-299,5.DOI:10.3969/j.issn.1674-5817.2012.04.008
牛血清白蛋白诱导免疫性心肌炎小鼠模型的建立
Establishment of Autoimmune Myocarditis Model Induced by Immunization of Mice with Bovine Serum Albumin
牛美真 1李继玲1
作者信息
- 1. 郑州市第二人民医院儿科,郑州450006
- 折叠
摘要
Abstract
Objective To approach feasible of autoimmune myocarditis induced by immunization of mice with bovine serum albumin, to proved a new animal model of autoimmune myocarditis. Methods Experimental group and control group were subcutaneously treated with 0.5 ml 10% bovine serum albumin and physiological saline respectively on day 0, 3, 5, and 10, and then related tests were conducted on day 14 and 28 to confirm if the model was established successfully. Results On day 14 after the first immunization with bovine serum albumin, all BALB/c mice from the experimental group were developed with myocardial interstitial lymphocytic infiltrates, focal myocardial necrosis and haemorrhages, accompanying with endocarditis and epicarditis. Under the electron microscope: It was found myocardial cell and mitochondria swelling, muscle fibers dissolved and lymphocyte infiltration. A high liter of myocardium creatase and more lymphocyte were detected in the immunized mice. On day 28 after the first immunization with bovine serum albumin, the inflammation and myocardial necrosis were not seriously, a same titer of myocardium creatase and less lymphocyte were detected in the immunized mice. Conclusion Autoinmune myocarditis can be induced by immunization of mice with bovine serum albumin, pathological changes of the animal model arre typical, the rate of colony is high.关键词
牛血清白蛋白/自身免疫/心肌炎Key words
Bovine Serum Albumin/ Autoimmune/ Myocarditis分类
生物科学引用本文复制引用
牛美真,李继玲..牛血清白蛋白诱导免疫性心肌炎小鼠模型的建立[J].实验动物与比较医学,2012,32(4):295-299,5.
