实验动物与比较医学2012，Vol.32Issue(4)：308-310,3.DOI:10.3969/j.issn.1674-5817.2012.04.011
顺铂致小鼠急性肾功能损伤模型的建立
Establishment of Acute Renal Impairment Model Induced by Cisplatin in Mice
杨喜花 1张生万 2任连生 3白喜花2
作者信息
- 1. 山西大学化学化工学院,太原030006
- 2. 山西省肿瘤研究所比较医学部,太原030013
- 3. 山西大学生命科学学院,太原030006
- 折叠
摘要
Abstract
Objective To establish a model of acute renal function impairment induced by cisplatin in mice. Method The mice were injected with 10 mg/kg cisplatin in the 1st day to establish acute renal impairment model. The mice in the control group were dosing commensurable 0.9% NaCl saline solution. The mice were taken eyeballs for blood collection after anesthesia by pentobarbitalin the 8th day, and the serum CRE, BUN levels were measured. Pathological analysis was taken to compare the changes of the renal tissues between model group and control group. Results CRE and BUN levels were raised obviously in the model group, renal index, body weight and immune organic weight were reduced obviously in the model group, at the same time, the liver index was raised in the model group. The result of the pathological analysis was showed that the degeneration and necrosis of renal tubular epithelial cells were observed in the model group, and there are some inflammatory infiltrates foci in renal mesenchymal cells in the model group. Conclusion Acute renal impairment model could be established by injected 10 mg/kg cisplatin in 32-40 g mice.关键词
顺铂/肾损伤/小鼠Key words
Cisplatin/ Acute renal impairment/ Mice分类
医药卫生引用本文复制引用
杨喜花,张生万,任连生,白喜花..顺铂致小鼠急性肾功能损伤模型的建立[J].实验动物与比较医学,2012,32(4):308-310,3.
