生命科学研究2012，Vol.16Issue(4)：283-286,4.
稳定表达人ASB12的C2C12细胞系的建立及鉴定
Establishment and Identification of a Stable Human ASB12-Expressed C2C12 Cell Line
摘要
Abstract
The human ASB12 (Homo sapiens ankyrin repeat and SOCS box containing 12) protein contains five ANK (ankyrin repeat sequence) domains and a SOCS (suppressor of cytokine signaling) box domain, belonging to the ASBs family. It was reported that ASB12 especially expressed in skeletal and cardiac muscles of adult tissues, which suggested that ASB12 closely associated with skeleton muscle development. To construct a stable ASB12-expressed C2C12 cell line, the fusion expression plasmid pCMV-tag2B-ASB12, which was identified by enzyme digestion and sequencing analysis, was transfected into C2C12 cell by cationic polymer. After screening culture by G418, the expression of ASB12 was detected by immunofluorescfence, RT-PCR and Western-blotting. The C2C12 cell line that expressing ASB12 stably was established successfully, which provide a cell model for studying the molecular function of ASB12 in skeleton muscle development.关键词
ASB12/ANK结构域/SOCS box结构域/C2C12稳定细胞系Key words
ASB12/ ANK domian/ SOCS box domain/ C2C12 stable cell line分类
生物科学引用本文复制引用
文斗斗,周军媚,赵明一,胡维新,吴秀山,王跃群..稳定表达人ASB12的C2C12细胞系的建立及鉴定[J].生命科学研究,2012,16(4):283-286,4.基金项目
湖南省自然科学基金资助项目(11JJ3028) （11JJ3028）
国家自然科学基金资助项目(81000035 （）
31071999 （）
30871340) （）
