生命科学研究2012，Vol.16Issue(4)：307-313,323,8.
ESPRG3多克隆抗体制备及初步功能研究
Polyclonal Antibodies Preparation and Functional Research of ESPRG3
摘要
Abstract
ESPRG3 is a gene related to hES cells. In order to investigate the function of ESPRG3, the mESPRG3 prokaryotic and hESPRd eukaryotic expression vector were constructed and polyclonal antibody was prepared. Molecular cloning and IPTG inducing were used to express and purify protein of ESPRG3 for the antibodies preparation. Western blot and imunohischemistry were used to identify the specificity of the antibody of Borealin. The recombinant proteins of ESPRG3 were found to show ability of binding DNA in vivo and in vitro with the ChlP-Chip assay and DNA select assay. These binding sites are overlapped with Alu and insu-lator elements. The function of it in ES cells may relate to these binding sites.关键词
ESPRG3/胚胎干细胞/多克隆抗体/染色质免疫沉淀(ChIP)Key words
ESPRG3/ embryonic stem cell/ polyclonal antibodies/ chromatin immunoprecipitation (ChIP)分类
生物科学引用本文复制引用
张前军,卢光琇..ESPRG3多克隆抗体制备及初步功能研究[J].生命科学研究,2012,16(4):307-313,323,8.基金项目
湖南省科技计划项目(2010FJ3026) （2010FJ3026）
