生命科学研究2012，Vol.16Issue(4)：314-318,5.
人孤雌胚胎干细胞在人包皮成纤维细胞饲养层上的生长状态
Growth of Human Parthenogenetic Embryonic Stem Cells on Feeder layer Derived from Human Foreskin Fibroblasts
王志强 1梁锐 2陈明清 1董坚 1邓俊 3陈天星 2李文亮 1文政琦1
作者信息
- 1. 昆明医科大学第一附属医院肿瘤科,中国云南昆明650032
- 2. 云南省第一人民医院病理科,中国云南昆明650032
- 3. 昆明医科大学第一附属医院男性科,中国云南昆明650032
- 折叠
摘要
Abstract
Human parthenogenetic embryonic stem cells (hPESCs) usually need the sustainment of feeder layer to keep stem cells characteristics ire vitro culture. Human foreskin fibroblasts (hFFs) were obtained by primary culture and prepared into the feeder layer of hPESCs. hPESCs were cultured and passaged on hFFs feeder layer in vitro. Morphology of cell growth was observed with inverted microscope. Alkaline phosphatase activity analysis, karyotype analysis and differentiation experiments in vivo were used to detect the biological char-acteristics and differentiated potency of hPESCs. Thereby whether hFFs can sustain hPESCs and keep hPESCs undifferentiated for long was explored. hFFs were obtained successfully by primary culture. Then hFFs were verified by morphology observation and immunocytochemical stain. Morphous of hPESCs on hFFs feeder layer is regular and it is not easy for hPESCs to differentiate. After subcultured successfully for more than 20 passages in vitro, hPESCs still maintained biological characteristics and normal karyotype. hPESCs can develop into teratomas containing three germ layers in nude mice. As a kind of feeder layer derived from human, hFFs can sustain the growth of hPESCs and keep hPESCs undifferentiated for long.关键词
人孤雌胚胎干细胞/人包皮成纤维细胞/饲养层/人胚胎干细胞/细胞培养Key words
human parthenogenetic embryonic stem cells/ human foreskin fibroblasts/ feeder-layer/ human embryonic stem cells/ cell culture分类
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王志强,梁锐,陈明清,董坚,邓俊,陈天星,李文亮,文政琦..人孤雌胚胎干细胞在人包皮成纤维细胞饲养层上的生长状态[J].生命科学研究,2012,16(4):314-318,5.
