食品工业科技2012，Vol.33Issue(21)：296-299,4.
HPLC法同时测定不同品种溪黄草中3种三萜类成分的含量
HPLC simultaneous determination of three triperpenoids components in Linearstripe Rabdosia Herb from different species
吴泽青 1帅欧 1林励 1邱戊盛 2欧先涛 2吴攀创2
作者信息
- 1. 广州中医药大学中药学院,广东广州510006
- 2. 广东万年青制药有限公司,广东汕头515064
- 折叠
摘要
Abstract
Objective:To establish an HPLC method for simultaneous determination of 2α-hydroxy-ursolic acid, oleanolic acid and ursolic acid in Linearstripe Rabdosia Herb from different species.Methods:The HPLC system consisting of Synergi 4u Fusion RP 80A C18 column (250mm ×4.6mm, 5μm, Phenomenex company) and a solution system of methanol-phosphoric acid(V:V = 9:1 )as the mobile phase with isocratic elution was adopted.The flow rate was set at 0.6mL·min-1 ,the detection wavelength was 210nm and the column temperature was 30℃.Results: The linear ranges of 2cc-hydroxy-ursolic acid ,oleanolic acid and ursolic acid were 0.111-1.111mg/mL( r =0.9999), 0.026- 0.256mg/mL( r = 0.9999) ,0.307-3.067mg/mL( r = 0.9999 ) respectively.Conclusion : The average recoveries ( n =6)were 97.72% (RSD = 1.24% ),97.08% (RSD = 1.62% ),97.46% (RSD = 1.25% ) respectively.The method was quick ,simple and accurate with good reproducibility and could be used for determination and quality control of the three main components in Linearstripe Rabdosia Herb.关键词
溪黄草/三萜/高效液相色谱法/测定Key words
Linearstripe Rabdosia Herb/triperpenoids/HPLC/determination分类
轻工纺织引用本文复制引用
吴泽青,帅欧,林励,邱戊盛,欧先涛,吴攀创..HPLC法同时测定不同品种溪黄草中3种三萜类成分的含量[J].食品工业科技,2012,33(21):296-299,4.
