水生生物学报2012，Vol.36Issue(4)：646-655,10.DOI:10.3724/SP.J.1035.2012.00646
四种细胞因子在中华蟾蜍脑中的分布
DISTRIBUTION ON FOUR TYPES OF CYTOKINES IN THE BRAIN OF THE TOAD,BUFO GARGARIIZANS
摘要
Abstract
Cytokines play an important role in the brain. A large number of experiments have showed that cytokines were expressed in the central nervous system of animals. In this paper, the strept avidin-biotin-peroxidase complex (SABC) immunohistochemical methods were applied to investigate the distribution of IL-lα, IFN-γ, NGF-β and TNF-α in the brain of adult toads, Bufo gargariizans. It found that quantities of IL-lα-like immunoreactive cells were detected and widely distributed in each region of the brain. IL-lα was mainly located in the bodies of positive cells. However, in the primordial hippoeampus, nucleus anterodorsalis tegmenti and nucleus anterovetralis tegmenti, positive cells' bodies and progresses were all labeled by IL-lα. Also, a large number of cells were stained by IFN-γ, these positive cells were observed in the primordial hippoeampus and septum of telencephalon, ventrolateral thalamus nucleus of thalamus, pre-optic area, suprachiasmatic nucleus and ventral infundibular nucleus of hypothalamus, nucleus anterodorsalis tegmenti, nucleus anterovetralis tegmenti, posterodorsal tegmental nucleus and posteroventral tegmental nucleus of mesencepha-lon, Purkinje cell layer of cerebellum, and reticular nuclei of medulla oblongata. Only in the primordial hippoeampus, nucleus anterodorsalis tegmenti, posterodorsal tegmental nucleus, Purkinje cell layer and reticular nuclei, progresses of these positive cells were IFN-γ-like immunoreactive. A few NGF-β-like immunoreactive cells were mainly observed in the preoptic area and suprachiasmatic nucleus of hypothalamus, nucleus anterovetralis tegmenti of mesencephalic teg-mentum, Purkinje cell layer of cerebellum, and reticular nuclei of medulla oblongata. NGF-β-like immunoreactivity was also presented in the progresses of these positive cells in the preoptic area, Purkinje cell layer and reticular nuclei. Several cells in the nucleus anterodorsalis tegmenti of mesencephalic tegmentum, and reticular nuclei and raphe nuclei of medulla oblongata, were labeled by TNF-α. And, their bodies and progresses were all TNF-α-like immunoreactive. Therefore, these results indicated that IL-lα and IFN-γ were widely expressed and may play an important and extensive role in the brain, but NGF-β and TNF-α were only presented in some specific regions and may take effect in these regions in the brain.关键词
白介素-1α/干扰素-γ/神经生长因子-β/肿瘤坏死因子-α/免疫组织化学/脑/中华蟾蜍Key words
IL-lα/ IFN-γ/ NGF-β/ TNF-α/ Immunohistochemistry/ Brain/ Bufo gargariizans分类
生物科学引用本文复制引用
杨乐乐,刘再群..四种细胞因子在中华蟾蜍脑中的分布[J].水生生物学报,2012,36(4):646-655,10.基金项目
安徽师范大学博士科研启动基金(160-750932) （160-750932）
生科院联合开放课题经费"安徽重要生物资源保护与利用研究"安徽省重点实验室科研基金 （）
"生物环境与生态安全"安徽省高校重点实验室基金资助 （）
