中国生态农业学报2012，Vol.20Issue(9)：1222-1229,8.DOI:10.3724/SP.J.1011.2012.01222
牛粪厌氧发酵酸化处理条件的优化
Optimization of cattle manure hydrolysis and acidification for methane production
摘要
Abstract
Although high lignocellulose content decreases acidification rale of anaerobic fermentation of cattle manure, acidification phase is critical in anaerobic fermentation of cattle manure. Cattle manure acidification conditions were investigated with the aim of optimizing methane production in a two-phased anaerobic process of cattle manure. The effects of acidification time. stirring frequency, feed solution concentration and urea addition for cattle manure methane production were studied in a two-phased anaerohic fermentation at 35 ℃. Single factor experiments showed that 96 h of acidification, 3 times in every 24 h (60 r·min-1, 1 min·time-1) of stirring frequency, 8.0% feed solution concentration and 1.28 g·L-1 urea addition were the optimum conditions. Based on single factor experiments, a series of central composite design experiments were conducted for optimum synthesis. Response surface methodology (RSM) with central composite design (CCD) was used to explore optimum conditions for methane production. The effects of three variables (acidification time, feed solution concentration and urea addition) were subsequently appraised. A regression model for methane production under the acidification conditions was established for analysis of interactions and significance in accordance with centra) composite design. Results showed that methane production was greatly influenced by feed solution concentration, urea addition and acidification time under at 35 ℃ and 3 times per every 24 h (60 r·min-1, 1 min·time-1) stirring frequency. The optimum acidification conditions occurred at the acidification time of 93.7 h, feed solution concentration of 8.3% and urea addition of 1.26 g·L-1. Under the optimum condition, methane content and total production increase by 14.3% and 44.7%. Also the removal rates of volatile solid (VS) and chemical oxygen demand (COD) increased by 41.8% and 33.9%, respectively. However, the contents of cellulose, hemicellulose and lignin were not affected by cattle manure acidification. The findings indicated that cattle manure acidification improved methane production, methane content, and VS and COD removal rates.关键词
牛粪/两相厌氧发酵/酸化处理条件/甲烷产量/响应面优化法Key words
Cattle manure, Two-phased anaerobic fermentation, Acidification condition, Methane production, Response surface methodology分类
资源环境引用本文复制引用
孙宇,付博锐,刘权,晏磊,王伟东,王彦杰..牛粪厌氧发酵酸化处理条件的优化[J].中国生态农业学报,2012,20(9):1222-1229,8.基金项目
"十二五"国家科技支撑计划项目(2012BAD12B05)、黑龙江省杰出青年科学基金(JC201002)、"十二五"黑龙江农垦总局重大科技专项(HNK11A-10-01)和黑龙江省科技厅科技攻关指导性项目(GZ11B108)资助 （2012BAD12B05）
