枯草芽孢杆菌D-29拮抗作用及其抗菌蛋白特性研究OACSTPCD
Antagonistic Effect of Bacillus subtilis D-29 and Characterization of its Antifungal Protein
枯草芽孢杆菌D-29是从地肤根内分离获得的一株生防细菌,为进一步了解D-29的生防特性,采用平板对峙法测定其抑菌谱并对其抑菌蛋白进行了研究.结果表明,该菌对番茄灰霉病菌、玉米大斑病菌等11种植物病原真菌均具有抑制作用,其中对玉米大斑病菌的抑菌效果最好,抑制率为54.6％确定80％饱和硫酸铵为最佳盐析饱和度,获得的粗提蛋白耐高温,对有机溶剂和紫外光照不敏感,耐酸不耐碱.
Biocontrol bacterial strain Bacillus subtilis D-29 was isolated from the root of Kochia scoparia. To gain valuable information for antagonistic effect, inhibition spectrum of D-29 was tested by plates inhibition methods and its antifungal protein was studied. D-29 showed antifungal activity against 11 pathogens. It showed the strongest antagonistic activity against Exserohilum turcicum and inhibitory rate reached 54. 6%. Crude protein was extracted wit…查看全部>>
谈静惠;高莹;程超;李宏;王美琴;郝晓娟
山西农业大学农学院,山西太谷030801山西农业大学农学院,山西太谷030801山西农业大学农学院,山西太谷030801山西农业大学生命科学学院,山西太谷030801山西农业大学农学院,山西太谷030801山西农业大学农学院,山西太谷030801
农业科技
枯草芽孢杆菌拮抗作用抑菌蛋白
Bacillus subtilis Antagonistic effect Antifungal protein
《山西农业大学学报（自然科学版）》 2012 (3)
224-227,4
国家自然基金青年基金(31000873)山西省青年基金(2010021031-1)山西农业大学博士后基金山西农业大学科技创新基金(2009019)山西省科技攻关项目(20080311025)
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