HeLa和SiHa细胞中Skp2、p27和C-myc的表达OA北大核心CSCDCSTPCD
Expressions of Skp2、p27 and C-myc in HeLa and SiHa Cells
目的:检测S期激酶相关蛋白2( Skp2)、C-myc、p27在宫颈癌HeLa及SiHa细胞系中的表达,明确这些指标在宫颈癌细胞系表达的意义.方法:通过细胞免疫组化、Western blot、RT-PCR技术分析Skp2、p27和C-myc在蛋白和mRNA水平表达的差异.结果:免疫组化显示:HeLa细胞中Skp2和C-myc表达强度高于SiHa细胞(P=0.032和P=0.026),而HeLa细胞中p27蛋白表达弱于SiHa细胞(P=0.035)…查看全部>>
Objective: To test the expressions of S-phase kinase-associated protein 2 (Skp2), C-myc and p27 in HeLa and SiHa cells from the levels of RNA and protein and to investgate the meaning of those indexes in cervical cancerous cells. Methods:We tested the expressions of Skp2, p27 and C-myc in HeLa and SiHa cell lines by immunohistochemistry, western blotting as well as semiquantitative PCR . Results: Immunohistochemistry results showed that the expressions of Sk…查看全部>>
薛翔;公丕军;范引侠
西安交通大学医学院第二附属医院,陕西西安710004西安交通大学医学院第二附属医院,陕西西安710004西安交通大学医学院第二附属医院,陕西西安710004
医药卫生
S期激酶相关蛋白2C-mycp27HeLa细胞SiHa细胞宫颈癌
S-phase kinase-associated protein 2 C-myc p27 HeLa cell SiHa cell Cervical cancer
《实用妇产科杂志》 2012 (8)
647-651,5
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