实用医学杂志2012，Vol.28Issue(13)：2162-2165,4.DOI:10.3969/j.issn.1006-5725.2012.13.025
经基因改造大肠杆菌DH5对裸鼠结肠癌肝转移灶的治疗效果
Gene modified Ecoli DH5 for the treatment of liver metastasis of colon cancer in nude mice
摘要
Abstract
Objective The operon of formic dehydrogenase gene which was sensitive to hypoxia, the promoter of lux gene which could perceive the difference of cell density, and diphtheria toxin gene were constructed by synthetic AND gate methods. Then all these kinds of gene were imported into auxotrophy Ecoli DH5α mutagenized by N-Methyl-N'-nitro-N-nitrosoguanidine (NTG), which was eventually injected into nude mice with colon cancer. And the efficacy of this gene modified Ecoli DH5α on the liver metastasis of colon cancer was observed. Methods The plasmid was constructed and imported into auxotrophy Ecoli DH5α, and then the Ecoli DH5α was injected into 10 nude mice to observe the toxicity, and was injected into 20 nude mice with liver metastasis of colon cancer to observe the efficacy. Results Ten mice injected with wild type of Ecoli DH5α were all dead, and only one was dead of those injected with auxotrophy Ecoli DH5α. The size of liver metastasis of colon cancer in mice injected with auxotrophs Ecoli DH5α which contained plasmid was smaller than that contained no plasmid. Conclusion The auxotrophs Ecoli DH5α is safe for normal mice, and has chemotaxis and anti-tumor effect toward cancer foci.关键词
结肠肿瘤/肝肿瘤/肿瘤转移/亚硝基胍/"与"门/DH5α/大肠杆菌Key words
Colonic neoplasms/ Liver neoplasms/ Neoplasm metastasis/ N-Methyl-N'-nitro-N-nitrosoguanidine/ AND gate/ DH5α/ Escherichia coli引用本文复制引用
姜宏华,周辉,张纪伟,莱代莉,子树明,徐佶,杜鹏,杨宝仁,崔龙..经基因改造大肠杆菌DH5对裸鼠结肠癌肝转移灶的治疗效果[J].实用医学杂志,2012,28(13):2162-2165,4.基金项目
国家自然科学基金项目(编号:30950017) （编号:30950017）
