实用医学杂志2012，Vol.28Issue(13)：2178-2180,3.DOI:10.3969/j.issn.1006-5725.2012.13.030
促红细胞生成素对阿霉素性心肌病大鼠心肌Bax和Bcl-2表达的影响
Erythropoietin effect on myocardial Bax and Bcl-2 expression for doxorubicin-induced cardiomyopathy of rats
陈兴 1王佩显 2许博裳1
作者信息
- 1. 510260,广州医学院第二附属医院老年病科
- 2. 300052,天津医科大学总医院心内科
- 折叠
摘要
Abstract
Objective To determine apoptotic protein expression of Bax and Bcl-2 of myocardium in rats of doxorubicin ( DOX) -induced cardiomyopathy, and to investigate protective mechanisms of erythropoietin (EPO) on DOX-induced cardiomyopathy. Methods Thirty-one Wistar rats were randomly divided into control group, DOX group and DOX+EPO group. After four weeks of drugs treatment, all rats were evaluated for general situation, echocardiography and histological analysis. Protein and mRNA expression of Bax and Bcl-2 were detected by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) respectively. Results There were 75% of rats with obvious ascites in DOX group. Rats in DOX group showed similar changes to those of DOX induced-cardiomyopathy in human by echocardiography and histological analysis. There was a significant improvement in cardiac function and myocardial fibrosis in DOX+EPO group compared to DOX group. Immunohistochemistry and RT-PCR revealed that compared to the DOX group, treatment with EPO did not decrease Bax protein and mRNA expression, but significantly increase Bcl-2 expression. Conclusions EPO may exert protective effects on DOX-induced cardiomyopathy, which may attribute to up-regulation of protein and mRNA expression of Bcl-2.关键词
心肌疾病/促红细胞生成素/阿霉素/凋亡/心肌纤维化Key words
Cardiomyopathies/ Erythropoietin/ Doxorubicin/ Apoptosis/ Myocardial fibrosis引用本文复制引用
陈兴,王佩显,许博裳..促红细胞生成素对阿霉素性心肌病大鼠心肌Bax和Bcl-2表达的影响[J].实用医学杂志,2012,28(13):2178-2180,3.
