天然产物研究与开发2012，Vol.24Issue(7)：945-950,6.
响应曲面法制备蛹虫草子实体酶解液的研究
Response Surface Optimization of Hydrolysis Processing for Cordyceps militaris
李晨曦 1姜兴华 1范青生 1余宙1
作者信息
- 1. 南昌大学 中德联合研究院,南昌330047
- 折叠
摘要
Abstract
In order to convert Cordyceps militaris which was a kind of food resouce into a convenient edible form,following experiments were carried out. First,papain was chosen as the enzyme to hydrolyze C. militaris from different kind of proteinase,in which degree of hydrolysis was designed as the target index. Response surface analysis ( RSA) was applied to optimize the conditions of enzymatic hydrolysis for C. militaris processing. According to the principle of Box-Behnken central composite design,enzymatic hydrolysis time,enzymeatic hydrolysis temperature,and enzyme dosage were chosen as factors to process the three-factor and three-level central composite design. By response surface analysis,the optimum conditions for enzymatic hydrolysis was obtained as follows: enzymatic hydrolysis time 11. 85 h, enzymatic temperature 60.92 ℃ ,enzyme dosage 1.02% .under which the highest hydrolysis degree reached with estimated value and verified value being 61.27% and 60.76% .respectively. These results demonstrated the validity of this hydrolysis process.
关键词
蛹虫草/酶解/木瓜蛋白酶/响应曲面法Key words
Cordyceps militaris/hydrolysis/papain/response surface analysis分类
医药卫生引用本文复制引用
李晨曦,姜兴华,范青生,余宙..响应曲面法制备蛹虫草子实体酶解液的研究[J].天然产物研究与开发,2012,24(7):945-950,6.
