西安交通大学学报（医学版）2012，Vol.33Issue(5)：568-571,4.
三阴性乳腺癌差异表达miRNA的筛选
MiRNA expression profile in triple-negative breast cancer
摘要
Abstract
Objective To screen and identify the miRNA differential expression profile in triple-negative breast cancer by the miRNA array technique. Methods Total RNA was extracted from 8 fresh breast tumor samples and normal adjacent tissues. The miRCURYTM LNA Array (v. 16.0) (Exiqon) was used to screen the differentially expressed miRNA. Real-time quantitative PCR was applied to verify the reliability of miRNA array results. Results Compared to normal adjacent tissues, 17 miRNAs were differentially expressed in triple-negative breast cancer tissues, including 7 up-regulated and 10 down-regulated miRNAs. The most significantly up-regulated miRNAs were miR-93 and miR-328, while miR-145 and miR-205 were significantly down-regulated. Conclusion We obtained miRNA differential expression profile of triple-negative breast cancer. The profile may be related to the pathogenesis and development of triple-negative breast cancer.关键词
miRNA/三阴性乳腺癌/miRNA芯片/实时定量PCRKey words
miRNA/triple-negative breast cancer/miRNA array/real-time quantitative PCR分类
医药卫生引用本文复制引用
赵晓艾,胡金华,赵新汉..三阴性乳腺癌差异表达miRNA的筛选[J].西安交通大学学报（医学版）,2012,33(5):568-571,4.基金项目
陕西省科学技术研究发展计划项目(社发攻关)(No.2010K14-01) （社发攻关）
