西安交通大学学报（医学版）2012，Vol.33Issue(5)：583-586,592,5.
宫颈癌组织表皮生长因子受体对TKI敏感性相关的基因突变研究
EGFR TKI-sensitive gene mutation in cervical cancer
薛琴琴 1张菊 2俞青苗 3金雯 1杨梅 1刘宁侠 1高艳娥1
作者信息
- 1. 西安交通大学医学院第二附属医院妇产科,陕西西安 710004
- 2. 榆林市第一医院妇产科,陕西榆林719000
- 3. 第四军医大学药学系基因所,陕西西安710032
- 折叠
摘要
Abstract
Objective To detect whether in epidermal growth factor receptor (EGFR) there exists mutation of exons 18, 19 and 21 associated with tyrosine kinase inhibitor (TKI) medication-sensitivity in cervical cancer patients for the purpose of providing evidence for targeting treatment of cervical cancer in the region. Methods Fresh tissue specimens were collected from 70 patients with cervical cancer and DNA was extracted from these specimens. Exons 18, 19 and 21 of EGFR were amplified with designed specific primers and DNA templates extracted from cervical cancer tissues. Then, the PCR products were sequenced on DNA sequencer, and the sequences were BLAST analyzed. Results No mutations were detected in exons 18, 19 or 21 of EGFR in the 70 human cervical cancer samples analyzed. Conclusion Mutations in exons 18, 19 and 21 of EGFR are very rare in cervical cancer.关键词
宫颈癌/表皮生长因子受体/基因突变/多聚酶链式反应/DNA测序Key words
cervical cancer/epidermal growth factor receptor/gene mutation/ polymerase chain reaction (PCR)/DNA sequencing分类
医药卫生引用本文复制引用
薛琴琴,张菊,俞青苗,金雯,杨梅,刘宁侠,高艳娥..宫颈癌组织表皮生长因子受体对TKI敏感性相关的基因突变研究[J].西安交通大学学报（医学版）,2012,33(5):583-586,592,5.
