西安交通大学学报（医学版）2012，Vol.33Issue(5)：598-601,4.
自发性高血压大鼠大、中动脉壁AT1和AT2受体表达的增龄性变化
Effects of aging on AT1 and AT2 expressions in the aorta and superior mesenteric artery of spontaneously hypertensive rats
摘要
Abstract
Objective To investigate whether aging leads to blood vessel remodeling in the aortic artery and superior mesenteric artery (SMA) walls of spontaneously hypertensive rats (SHRs) with the changes of angiotensin Ⅱ receptor-1 (ATI) and angiotensin Ⅱ receptor-2 (AT2) in local artery walls. Methods A total of 60 male SHRs aged 7, 26 and 51 weeks were divided into young, adult, and aged groups (20 in each group). After 1-week normal diet, the systolic blood pressure of the SHRs was measured and analyzed by a tail-cuff method. The expressions of ATI and AT2 in arteries were detected by immunohistochemical SABC staining in paraffin sections. The degree of the sediment of collagen fibers was detected using Mallory three-color staining. Results Blood pressure of the SHRs significantly increased with aging [(151±26)mmHg vs. (166±9.0)mmHg vs. (180±13)mmHg, P<0.05]. Aging induced a significant increase of sediment of collagen fibers in the SHRs (IA values in aortic artery 416 ±43 vs. 483±74 vs. 553±83; in SMA 387 ± 46 vs. 425 ± 67 vs. 468±81, both P<0.05). ATI and AT2 were expressed significantly higher with aging in the aortic artery (gray degree of ATI: 159±7.0 vs. 150±8.7 vs. 139±8.9; AT2: 187±6.9 vs. 181±9.5 vs. 169±10, both P<0.05), which was the same as in SMA (ATI: 163±11 vs. 112 ±11 vs. 128±18; AT2, 188±11 vs. 171 ±15 vs. 171 ± 13, both P<0.05). Aging also induced the increase of AT1/AT2 ratio in aged SMAs (0.85±0.07 in young rats vs. 0.71±0.08 in old ones, P<0.01), and in the aortic artery (0.86±0.01 in young rats vs. 0.78±0.08 in old ones, P<0.05). Conclusion Sediment of collagen fibers induced the changes of ATR expression profiling and function in local artery walls may be one of the mechanisms of artery remodeling and increasing SBP induced by aging.关键词
自发性高血压大鼠/AT1型受体/AT2型受体/高血压/血管重塑/增龄Key words
spontaneously hypertensive rat/angiotensin Ⅱ type 1 receptor/angiotensin Ⅱ type 2 receptor/hypertension/vessel remolding/aging分类
医药卫生引用本文复制引用
吕颖,孙超峰,张军波,殷艳蓉,郝丽娜,高海燕..自发性高血压大鼠大、中动脉壁AT1和AT2受体表达的增龄性变化[J].西安交通大学学报（医学版）,2012,33(5):598-601,4.基金项目
国家自然科学基金资助项目(No.39870333) （No.39870333）
