西安交通大学学报（医学版）2012，Vol.33Issue(5)：658-660,封3,4.
姜黄素联合顺铂对人卵巢癌SKOV-3细胞增殖的抑制作用
Anti-proliferation effect of curcumin combined with cisplatin on human ovarian cancer SKOV-3 cells in vitro
范引侠 1王艳华 2邢兰瑛1
作者信息
- 1. 西安交通大学医学院第二附属医院妇产科,陕西西安710004
- 2. 北京市丰台区南苑医院妇产科,北京100076
- 折叠
摘要
Abstract
Objective To investigate the anti-proliferation effect of curcumin and curcumin combined with cisplatin on human ovarian cancer cell line SKOV-3 in vitro. Methods Human ovarian cancer SKOV-3 cells were cultured and treated with different concentrations of curcumin (5, 10, 20, 40 and 80 μmol/L), cisplatin (10 μmol/L), or curcumin combined with cisplatin in different groups. The proliferation of SKOV-3 cells was detected by MTT assay. Flow cytometry was used to analyze the apoptosis and cell cycle distribution. Results MTT assay showed that curcumin of different concentrations could significantly inhibit the proliferation of SKOV-3 cells in time-dependent and dose-dependent manners (P<0.05). Compared with that in single drug groups, the suppression in combination treatment group was significantly higher (P<0.05). Flow cytometry showed that curcumin could arrest SKOV-3 cells in G2/M phase. Furthermore, curcumin could induce apoptosis. With the increased concentration of curcumin treatment, the apoptosis rate increased. Conclusion Curcumin can inhibit the growth of SKOV-3 cells. In a certain range, the effect is in dose- and time-dependent manners. With curcumin combined with cisplatin, the effect could be enhanced in a synergistic manner.关键词
姜黄素/顺铂/卵巢癌SKOV-3细胞/卵巢癌/细胞凋亡Key words
curcumin/cisplatin/SKOV-3 cell/ovarian cancer/cell apoptosis分类
医药卫生引用本文复制引用
范引侠,王艳华,邢兰瑛..姜黄素联合顺铂对人卵巢癌SKOV-3细胞增殖的抑制作用[J].西安交通大学学报（医学版）,2012,33(5):658-660,封3,4.
