陕西科技大学学报（自然科学版）2012，Vol.30Issue(4)：61-63,80,4.
苦参愈伤组织诱导及继代培养条件研究
Study on the induction and subculture condition of callus of Sophora flavescens Ait
刘瑶 1齐香君 1李雅 1李军2
作者信息
- 1. 陕西科技大学生命科学与工程学院,陕西西安 710021
- 2. 中国药科大学国际医药商学院,江苏南京211198
- 折叠
摘要
Abstract
[Objective] The research studied the condition of induction and subculture callus of Sophora flavescens Ait. , to provide a new source for matrine and oxymatrine accumulation. [Method] The influence on induction callus were researched with the different explants and combinations of hormone. Meanwhile, the best proliferation conditions of callus were studied with different combinations of hormone. [Result] The inducting rates was 94. 12%, 74.19%, 35. 71%, 23. 08% with the young bud, roots, stems,leaves respectively on the medium of MS+1. 0 mg/L 2.4D+1. 0 mg/L 6-BA +1. 0 mg/L NAA. The best proliferating culture medium of callus was MS+0. 5 mg/L 2,4D+O. 5 mg/L 6-BA + 0. 5 mg/L NAA,and the increment rate was 25. 3% in this conditions. [Conclusion] Young buds were the best explant in the studies. Different combinations of hormone had a little impact on the induction of callus, but it had anotable effect on the increment rate of callus, especially NAA.关键词
苦参/愈伤组织/苦参碱/氧化苦参碱Key words
Sophora flavescens Ait. / callus/ matrine/ oxymatrine分类
农业科技引用本文复制引用
刘瑶,齐香君,李雅,李军..苦参愈伤组织诱导及继代培养条件研究[J].陕西科技大学学报（自然科学版）,2012,30(4):61-63,80,4.
