新疆农业科学2012，Vol.49Issue(7)：1190-1196,7.DOI:10.6048/j.issn.1001-4330.2012.07.003
玉米ZmCPK12基因真核表达载体的构建及转化
Construction and Transformation of Eukaryotic Express Vector of ZmCPK12 Gene from Maize
摘要
Abstract
[ Objective ] CDPK only found in plants and lower animals, is a protein kinase dependent on Ca + but not on CaM and the phospholipid, or a class of calmodulin domain protein kinase. The purpose of this research is to provide the basis for understanding the function of the CDPK family genes from maize in the resistance. [ Method ] Construction of ZmCPKll eukaryolic expression vector is an important way to understand the gene function in maize. In this paper, ZmCPKVZ of 1533 bp was cloned by using RT - PCR, and was digested by the restriction of enzymes Sail and Notl, and then it was connected with pREP -5N to obtain the recombinant eukaryotic expression plasmid ZmCPKll -5/Y. [Result] Restriction enzyme digestion and sequencing methods were identified by colony PCR, and the results showed that the recombinant eukaryotic expression the plasmid ZmCPKll - 5/V was successfully constructed. Preparation of yeast competent, by electric shock, the recombinant plasmid was transformed into yeast. Yeast drop - PCR results showed that electroporation was successful. [ Conclusion ] Construction of ZmCPKVl gene eukaryotic expression vector and transformation were successful.关键词
玉米/基因/真核表达载体/重组质粒构建Key words
maize/ gene/ eukaryotic expression vector/ construction of recombinant plasmid分类
农业科技引用本文复制引用
张毓露,郝晓燕,足木热木,刘小利,陈果,陈勋基,黄全生..玉米ZmCPK12基因真核表达载体的构建及转化[J].新疆农业科学,2012,49(7):1190-1196,7.基金项目
新疆维吾尔自治区高技术研究发展计划项目(201011109) （201011109）
