新疆农业科学2012，Vol.49Issue(9)：1594-1599,6.DOI:10.6048/j.issn.1001-4330.2012.09.005
棉纤维总RNA提取方法的比较与分析
Comparison and Analysis of Total RNA Isolation Method from Cotton Fiber
摘要
Abstract
[ Objective ] This study aimed to screen total RNA extraction method for cotton fiber and improve it to meet the transcriptome expression profiling sequencing requirements. [ Method ] Three methods of extraction of cotton fiber total RNA were used, that is, modified hot borate method, the Trizol method and centrifugal column kit method. [ Result]The quality of total RNA extracted by the modified hot borate method is the best, while the improvement in this method is more suitable for the extraction of total RNA of different periods of the same species and different varieties of cotton fiber of the same period. [ Conclusion ] Comparison of cotton fiber total RNA extraction methods, the total RNA extracted by comparing three kinds of cotton fiber, quality of total RNA extracted by the modified hot borate method is the best, and improvement of it could better meet the transcriptome expression profiling sequencing requirements.关键词
棉纤维/总RNA/表达谱/热硼酸法Key words
cotton fiber/ total RNA/ expression profile/ hot - borate method分类
农业科技引用本文复制引用
马顺尧,曲延英,高文伟,陈磊,郑炜佳,陈全家..棉纤维总RNA提取方法的比较与分析[J].新疆农业科学,2012,49(9):1594-1599,6.基金项目
转基因生物新品种培育科技重大专项(2011ZX005-004) （2011ZX005-004）
国家自然科学基金(31060151) （31060151）
新疆维吾尔自治区自然科学基金(2010211a16) （2010211a16）
