厦门大学学报（自然科学版）2012，Vol.51Issue(5)：911-917,7.
高山被孢霉原生质体转化及延长酶基因同源表达的研究
Study on Protoplast Transformation of Mortierella alpina and Its Homologous Expression of Elongase Gene
摘要
Abstract
In order to improve the production of Arachidonic acid (ARA) .the protoplasts from Mortierella alpina were transformed with re-combinant pD4 plasmid carrying the GLELO gene (elongase gene) in PEG system to study its homologous expressioa The results showed that the best transformation system for preparing protoplasts sorbitol as osmotic stabilizer with 50 mmol/L Ca2+ and PEG4000 as the trans-formant medium, which was incubated at 25 ℃ for 15 min. After regenerating, the highest transformation ratio of 1. 8 μg-1 was got. The results in shaking culture showed that ARA yield was almost improved by 30% in the recombinant Mortierella alpina than the original strain. It's suggested that it will be useful for studying on the improvements of traits in Mortierella alpina and the genetic transformation in other fungi.关键词
高山被孢霉/花生四烯酸/原生质体转化/延长酶基因/聚乙二醇Key words
Mortierella alpina /arachidonic acid/protoplast transformation/elongase gene/PEG分类
生物科学引用本文复制引用
张长杰,凌雪萍,曾思钰,卢英华..高山被孢霉原生质体转化及延长酶基因同源表达的研究[J].厦门大学学报（自然科学版）,2012,51(5):911-917,7.基金项目
国家自然科学基金项目(31071488) （31071488）
福建省科技厅重大专项(2010NZ0001-4) （2010NZ0001-4）
