畜牧兽医学报2012，Vol.43Issue(8)：1215-1221,7.
自由基吸收类抗氧化剂对延边黄牛体细胞克隆重构胚发育的影响
Effects of Free Radical Absorptive Antioxidants on the Development of Somatic Cell Nuclear Transfer Yanbian Yellow Cattle Embryos
摘要
Abstract
In order to improve the development efficiency of somatic cell nuclear transfer embryos of Yanbian Yellow cattle, free radical absorptive antioxidants, such as vitamin E, EGCG and sodium selenite, were added in culture medium in vitro with different concentrations for observing the development of the reconstructed embroys. Oocytes were cultured 20-22 h in maturation medium, oocytes with first polar body were chose for enucleation, injection, integration and activation, then restructured embryos were cultured in in vitro culture mediums with antioxidants with different concentrations. On the cleavage rate, in VE study, results showed that 100 μmol o L-1 group was significantly higher than those of other concertrations(84. 58% vs 57. 64%, 70. 87%, 67. 64%); there ' were no significant differences between 50 and 200 μmol o L-1 groups, but they were both significantly higher than that of 0 μmol o L-1 group(70. 87%, 67. 64% vs 57. 64%); In EGCG study, results showed that 7. 5 and 15 μmol o L-1 groups were significantly higher than that of 0 μmol o L -1 group(79. 47%, 81. 67% vs 69. 47%)and there were no significant differences hetween other groups;In SS study,5 and 0 ng o mL-1 group were significantly higher than 10 ng o ml/-1 group (80.44% vs 70. 27%,67. 16%) while there were no significant differences with 2. 5 ng o mL-1 group(80. 44% vs 73. 92%) , and there were no significant differences between other groups. On the Blastocyst rate,in VE study,results showed that 100 μmol o L-1 group was significantly higher than those of other concertrations(26. 36% vs 13. 04%,18. 08%,17. 26%);In EGCG study, results showed that 15 μmol o L-1 group was significantly higher than 0 and 30 μmol o L-1 groups (24. 86% vs 8. 99%, 12. 10%)and there was no significant differences with 7. 5 μmol o L-1 group;In SS study, 5 ng o mL-1 group was significantly higher than that of 0 and 10 ng o mL-1 groups (23. 31% vs 10. 32%,12. 65%);2. 5 ng o mL-1 group was significantly higher than that of 0 and 10 ng o mL-1 groups(19. 97% vs 10. 32%, 12. 65%) while there were no significant differences between other two groups. Adding 100 μmol o L-1 vitamin E,15 μmol o L-1 EGCG or 5 ng o mL-1 sodium selenite alone in vitro into culture medium can enhance the early development rate of Yanbian Yellow cattle restructured embryos in vitro.关键词
延边黄牛/体细胞克隆/抗氧化剂Key words
Yanbian Yellow cattle/somatic cell clone/antioxidant分类
农业科技引用本文复制引用
尹多,朱玉霞,柳海星,李钟淑,方南洙..自由基吸收类抗氧化剂对延边黄牛体细胞克隆重构胚发育的影响[J].畜牧兽医学报,2012,43(8):1215-1221,7.基金项目
国家自然科学基金(30860184) （30860184）
延边大学211工程三期立项项目 （）
