畜牧兽医学报2012，Vol.43Issue(8)：1281-1286,6.
应用噬菌体展示技术筛选兔出血症病毒抗原模拟表位
Screening of Antigen Mimotopes of Rabbit Hemorrhagic Disease Virus by Phage Display Technology
摘要
Abstract
The monoclonal antibody (McAb) A3c against rabbit hemorrhagic disease virus had been used as solid-phase selective molecule to screen the epitope of RHDV by phage display technology. McAb A3c was coated on the solid-phase and then three rounds of biopanning were carried out; 25 phages were selected and amplified to be identified by ELISA, and the positives were sequenced; the serum of mice immunized three times was prepared to determine the immunogenic-ity of the epitope. The results showed that the specific phages were enriched effectively after three rounds of biopanning; 19 of 25 phages were positive. The sequence analysis of the positive clones showed that highly homogenous sequence (GTDDMDPGTTAA) comparing to antigen was got, which is the antigen mimotopes of RHDV. The sequence DXXDP was the core amino acids in the epitope. In addition, the serum of mice reacted with antigen well and so did positive phages with hyper-immune serum of rabbit against RHDV, which indicated that the epo'tipe had good immunogenicity and reactionogenicity. This study provide theoretical basis for studying the epitope of RHDV and new vaccines.关键词
兔出血症病毒/单克隆抗体/噬菌体展示技术/模拟表位Key words
rabbit hemorrhagic disease virus/ monoclonal antibody/ phage display technology/ mimotopes分类
农业科技引用本文复制引用
杨廷亚,王芳,姜平,胡波,范志宇,魏后军,薛家宾..应用噬菌体展示技术筛选兔出血症病毒抗原模拟表位[J].畜牧兽医学报,2012,43(8):1281-1286,6.基金项目
现代农业产业技术体系建设专项资金资助(CARS-44) （CARS-44）
