畜牧兽医学报2012，Vol.43Issue(8)：1287-1291,5.
脂质体包被鸡IL-18可增强新城疫活疫苗的免疫效果
Effect of Mature Chicken Interleukin-18 Protein on Its Immunoenhancement on Newcastle Disease Vaccine
摘要
Abstract
The liposome-encapsulated mChIL-18 was firstly applied on its immunoenhancement for Newcastle disease vaccine with different concentrations. In the experiment, one-day-old SPF chickens were randomly divided into six groups, the blank control group, the NDV vaccine group, the NDV vaccine plus 0. 2 mL mChIL-18 group, the NDV vaccine plus 0. 1 mL mChIL-18 group, the NDV vaccine plus 0. 3 mL mChIL-18 group and the NDV vaccine plus vector control group. All groups were immunized on the seven days old. After that, peripheral blood was collected with anticoagulation, and the antibody level, CD4+ and CD8+ T lympholeukocytes were detected by HI and FACS at each week, respectively. Standard Virulent Strain F48E9 (100 times ELD50 per chicken) was given to the chickens at last vaccination. The results indicated that the best concentration of mChIL-18 liposomes is 0. 2 mL per chicken. The mChIL-18 liposomes can not only enhance the cellular immune response significantly, but also enhance the humoral response, improving the protective rate of ND vaccine.关键词
mChIL-18/脂质体/新城疫疫苗/免疫增强Key words
mChIL-18/ liposomes/ Newcastle disease vaccine/ immunoenhancement分类
农业科技引用本文复制引用
张海滨,周明东,周霞,胡敬东,赵宏坤..脂质体包被鸡IL-18可增强新城疫活疫苗的免疫效果[J].畜牧兽医学报,2012,43(8):1287-1291,5.基金项目
国家“十二五”科技支撑项目(2012BAK17B05) （2012BAK17B05）
国家肉鸡产业技术体系建设项目 （）
山东省科技支撑计划项目(2007GG20009001) （2007GG20009001）
