畜牧兽医学报2012，Vol.43Issue(8)：1317-1323,7.
ALV-J诱发鸡成红细胞白血病的临床病例分析
Clinical Cases Analysis of Avian Leukosis Virus Subgroup J Induced Erythroblastosis in Chicken
摘要
Abstract
Erythroblastosis was diagnosed in two flocks of Chinese local breed and one flock of layer chicken during 2010 and 2011. The age of birds was 110-day, 90-day and 20-day, respectively. The main symptoms were characterized by emaciation, depressed and lethargy. At necropsy, numerous similar nodular lesions and resembling tumors were observed in one or more tissues. Some organs showed enlargement. A large number of erythrocytoblasts were observed in blood and bone marrow smears. Erythrocytoblasts were larger, basophilic cytoplasm and round nucleus. Histological examination showed that erythrocytoblasts were crowded in both blood vessels and mesenchym. Mitosis of erythrocytoblasts was often seen. No evidences showed Marek's disease and reticuloendotheliosis, poisoning and metabolic diseases in these sick chickens. The PCR-based diagnosis showed that ALV-J specific fragment of 924 bp was obtained, rather than ALV-A and ALV-B. Immunohistochemistry results showed that a diffuse presence of ALV-J antigen in various tissues. Taken all data together, it demonstrated that erythroblastosis in the three flocks was induced by ALV-J. Erythroblastosis induced by ALV-J was found first time in China flocks. The etiology and mechanism of tumor spectrum expanded of ALV-J need to be further studied.关键词
J亚群禽白血病病毒(ALV-J)/成红细胞白血病(EB)/PCR/免疫组化Key words
avian leukosis virus subgroup J/ erythroblastosis/ PCR/immunohistochemistry分类
农业科技引用本文复制引用
姜艳萍,王玥,于琳琳,蔡黎明,王真真,徐晴晴,王桂花,成子强..ALV-J诱发鸡成红细胞白血病的临床病例分析[J].畜牧兽医学报,2012,43(8):1317-1323,7.基金项目
国家自然科学基金(31072096) （31072096）
山东省科技攻关(2010GNC10943) （2010GNC10943）
