畜牧兽医学报2012，Vol.43Issue(8)：1324-1329,6.
猪肺炎支原体P216基因片段的表达及黏附活性研究
Expression of P216 Gene Fragment from Mycoplasma hyopneumoniae and Research on Adhesion Activity
摘要
Abstract
This experiment was conducted to study the adhesion activity of P216 protein and establish the model of adhesion protein of Mycoplasma hyopneumoniae (Mhp). According to analysis, the fragment of P216 gene with hydrophilicity, antigenicity and good adhesion was chose. P216 gene fragment was amplified by PCR from Mhp NJ strain and inserted into expression vector pET-32a( + ) , and the recombinant plasmid pET-32a( + )/P216 was constructed. After IPTG induction, the immunological and adhesion activity of the recombined protein was detected by Western blot and indirect immunofluorescence assay. The results showed that, the PCR product of the target gene was 1 636 bp, the molecular weight of recombinant protein was 80. 1 kDa by SDS-PAGE, and Western blot results showed that recombinant protein had satisfactory immuno-genicity. Indirect immunofluorescence assay showed that the recombinant protein could produce occupied inhibition to the Mhp adhering with SJPL cells. These results indicated that the P216 protein had good adhesion activity, and could adhere SJPL cells. It provides new ideas for re-search of the other adhesions from Mycoplasma hyopneumoniae.关键词
猪肺炎支原体/P216基因片段/黏附活性Key words
Mycoplasma hyopneumoniae / F216 gene fragment/ adhesion分类
农业科技引用本文复制引用
杜海霞,刘茂军,冯志新,熊祺琰,白方方,王海燕,邵国青..猪肺炎支原体P216基因片段的表达及黏附活性研究[J].畜牧兽医学报,2012,43(8):1324-1329,6.基金项目
农业科技成果转化资金(2009GB2C100129) （2009GB2C100129）
江苏省农业科技自主创新资金(cx(10)215) （cx(10）
