畜牧与兽医2012，Vol.44Issue(10)：25-29,5.
崇明白山羊gdf-9基因多态性与产仔率的关联分析
Polymorphism of growth differentiation factor 9 gene and its relationship with litter size of Chong-ming white goats
摘要
Abstract
Chong-ming white goats which lived in Chong-ming island of Shanghai has a characteristic of high prolificacy. For the purpose of exploring its hereditary basis, the polymorphism of gdf-9 gene in chong-ming white goats was analyzed by PCR-SSCP. The results indicated that there were three mutation points in the cds of gdf-9 gene: one single nucleotide was mutated (A-G) at +183bp of exon 1 , two nucleo-tides were found at +1188bp and +1189bp which made the amino acid turn from valine to isoleucine, and the Gil88A had not been reported in other goats. Among the 75 goats, the gene frequencies of G ( G) , G ( A) and A (A) allelotypes of Gl 188A were 86. 67% , 13. 33% and 0, respectively. And the allelotypes G (G) , G ( A) and A ( A) of G1189A had a frequencies of 60. 00% , 40.00% and 0% ; for G1188A mutation, the average litter size of G (G) was higher than G (A) , P>0. 05; G ( A) of G1189A mutation had bigger average size than that of G ( G). In brief, by comparing the avrarage litter size, the results were AC>AA>AB ( P>0. 05 ). Therefore, the results showed that the different genotypes of gdf-9 gene could not significantly affect the litter size in the Chong-ming goat breed.关键词
崇明白山羊/gdf-9基因/基因多态性/高繁殖率/育种Key words
Chongming white goat/ gdf-9/ high prolificacy/ breeding分类
农业科技引用本文复制引用
任丽娜,刘思国,胡大伟,兰翀,谭婷婷,王敬军,陈建泉,马恒东,成国祥..崇明白山羊gdf-9基因多态性与产仔率的关联分析[J].畜牧与兽医,2012,44(10):25-29,5.基金项目
国家863计划(2008AA101001) （2008AA101001）
上海市优秀技术带头人计划(11XD1422000) （11XD1422000）
上海市科委研发基地建设项目(10dz2251700). （10dz2251700）
