信阳师范学院学报（自然科学版）2012，Vol.25Issue(4)：490-494,5.DOI:10.3969/j.issn.1003-0972.2012.04.017
家蚕新的非编码RNA功能初探
Functional Dissection of Novel Non-coding RNA in Silkworm Bombyx mori
摘要
Abstract
The cDNA library of 50-500 nt ncRNAs in Bombyx mori were constructed, and a small RNA Bm-86 accumulated more in larva and pupa during development was found. To further dissect the function of this ncRNA, bioin-formatics analysis, northern blot and RT-PCR were taken out. Results showed that Bm-86 was an H/ACA box small nucleolar RNA, located in the second intron of eIF5A, with no predicted pseudouridylation modification site in rRNAs, snRNAs and tRNAs of B, mori. Moreover, a putative promoter were located 263 bp upstream of Bm-86, which indicated that Bm-86 might be transcribed independently. Further analysis showed that the expression of Bm-86 exhibited negative correlation with its host gene eIF5A, notably in silkgland, which indicated that Bm-86 might be involved in the development of silkgland through regulating the expression of eIF5A.关键词
家蚕/非编码RNA/丝腺Key words
Bombyx mori/ non-coding RNA/ silkgland分类
生物科学引用本文复制引用
张琨,李丹丹,刘宗才,阚云超,刘卫群..家蚕新的非编码RNA功能初探[J].信阳师范学院学报（自然科学版）,2012,25(4):490-494,5.基金项目
973国家重大科技专项子项目(2009CB825400) （2009CB825400）
国家自然科学基金项目(31172158) （31172158）
