云南化工2012,Vol.39Issue(5):45-47,3.
高效液相色谱法测定白酒中纽甜含量
Determination of Neotame in Distilled Spirit by High Performance Liquid Chromatography
彭飞进1
作者信息
- 1. 云南省大理白族自治州质量技术监督综合检测中心,云南大理671000
- 折叠
摘要
Abstract
A method for determination of Neotame in Distilled Spirit by high performance liquid chromatography (HPLC) has been established. Ethanol is removed by heating with boiling water and constant volume to the corresponding volume with water. Neotame is determined by HPLC with the factors such as the detector DAD, the column of Agilent Eclipse XDB-C18 (4.6 mm × 250 mm, 5 um), mobile phase ratio (Vacetonitrile : Vion pair buffer solution = 25:75), sample size 20 uL, the temperature of the column at 30℃ and testing wavelength at 218 nm. It is quantified using external standard. The recoveries of the method with satisfactory precision ranged from 97% to 102%. This method is simple and suitable for the determination of Neotame in distilled Spirit.关键词
高效液相色谱法(HPLC)/纽甜/白酒。Key words
High Performance Liquid Chromatography (HPLC)/Neotame/distilled spirit分类
化学化工引用本文复制引用
彭飞进..高效液相色谱法测定白酒中纽甜含量[J].云南化工,2012,39(5):45-47,3.